Propagation of (Bupleurum gibraltaricum lam.) by tissue culture technique.

Abstract

This study was carried out at the plant tissue culture laboratories in the Biotechnology Research Centre of Tripoli Libya in collaboration with the Faculty of Agriculture University of Tripoli in order to find out the best protocol for propagation of(Bupleurum gibraltaricum lam.) through tissue culture technique . The results indicated that the best concentration of sodium hypochlorite solution for surface sterilization was 2.5% for 15 minutes and the best period for collection shoots source of explants node cutting was in March. The results also revealed that the most suitable  media for shoots explants production was (MS) medium supplemented with 1mg/l benzyl adenine (BA) which gave the heights length of the shoots and number of leaves per shoots. Moreover the height rooting rate, root length and root number were obtained in (MS) medium supplemented with 6mg/l of indole butyric acid. Furthermore the results from this study showed that percentage of plants survival after acclimatization was up to 70%.

Key Words: Bupleurum gibraltaricum, tissue culture, benzyl adenine, indole butyric acid and Kinetin